Evie Papathanassiou studied at the Municipal Conservatoire of Larissa, the New Conservatoire of Thessaloniki and the Department of Music Studies of the Aristotle University in Thessaloniki. She obtained the cello diploma from the State Conservatoire of Thessaloniki, where she studied with Dimitris Patras. She continued her studies at Trinity College of Music in London with Derek Aviss (postgraduate diploma with distinction) and in 1998 obtained a Master in Performance and Related Studies from the University of London. She has performed solo and chamber music in Greece, England and Prague, and was awarded first prize in the string category of the Anglo-Czechoslovak Trust competition. She has played with various chamber music groups and most Greek orchestras. Since 2001 she has been a permanent member of the Athens State Orchestra.